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random hexanucleotide primer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher random hexanucleotide primer
    Random Hexanucleotide Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexanucleotide+primers/random+hexanucleotide+primer/pmc10928104__41419_2024_6576_MOESM3_ESM-90-12-18
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The Molecular Mechanisms of Oleanane Aldehyde-β-enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells.
    Article Snippet: .. RNA reverse transcription was carried out with random hexanucleotide primers and M-MLV reverse transcriptase (Thermo Fisher Scientific, Waltham, MA, USA). .. Then, real-time PCR was performed on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: RNA Binding Protein CUGBP2/CELF2 Mediates Curcumin-Induced Mitotic Catastrophe of Pancreatic Cancer Cells
    Article Snippet: Results were analyzed with ModFit LT software (Verity Software House, Topsham, ME). .. Total RNA isolated from MiaPaCa-2, Pan02 cells and tumor xenografts using TRIZOL reagent was reverse transcribed with Superscript II reverse transcriptase in the presence of random hexanucleotide primers (all from Invitrogen, Carlsbad, CA). .. Complementary DNAs were then used for Real time PCR using Jumpstart Taq DNA polymerase (Sigma Chemical Co, St. Louis, MO) and SYBR Green nucleic acid stain (Molecular Probes, Eugene, OR).

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals.
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [31], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [28,31]. ..

    Article Title: Lipocalin-2 Deficiency Diminishes Canonical NLRP3 Inflammasome Formation and IL-1β Production in the Subacute Phase of Spinal Cord Injury.
    Article Snippet: Afterwards, purity and RNA concentration were measured using a NanoDrop 1000 device (PeqLab). .. For cDNA synthesis, 1 μg of RNA was used, and reverse transcription was performed using an MMLV reverse transcriptase kit and random hexanucleotide primers (Invitrogen, Braunschweig, Germany). .. Semiquantitative real-time PCR (qRT-PCR) was performed using SYBR Green (Bioline, Braine-Álleud, Belgium, QT615-05) in the CFX Connect qPCR detection system (Bio-Rad, Feldkirchen, Germany).

    Article Title: Nanoparticle-based delivery of siDCAMKL-1 increases microRNA-144 and inhibits colorectal cancer tumor growth via a Notch-1 dependent mechanism
    Article Snippet: .. Total RNA isolated from tumor xenografts and HCT116 cells was subjected to reverse transcription using SuperscriptTM II RNase H-Reverse Transcriptase and random hexanucleotide primers (Invitrogen, Carlsbad, CA). .. The complementary DNA (cDNA) was subsequently used to perform real-time polymerase chain reaction (PCR) by SYBRTM chemistry (SYBR Green I, Molecular Probes, Eugene, OR) for specific transcripts using gene-specific primers and JumpStartTM Taq DNA polymerase (Sigma-Aldrich).

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [ ], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [ , ]. ..

    Article Title: Small molecule kinase inhibitor LRRK2-IN-1 demonstrates potent activity against colorectal and pancreatic cancer through inhibition of doublecortin-like kinase 1
    Article Snippet: .. First strand cDNA synthesis was carried out using SuperScript II Reverse Transcriptase and random hexanucleotide primers (Invitrogen). .. The complementary DNA was subsequently used to perform RT-PCR on an iCycler IQ5 Thermal Cycler (BioRad) using SYBR Green (Molecular Probes) with gene-specific primers and JumpStartTM Taq DNA polymerase (Sigma).

    Article Title: Ehlers-Danlos Syndrome, Hypermobility Type, Is Linked to Chromosome 8p22-8p21.1 in an Extended Belgian Family
    Article Snippet: Total RNA was isolated from cultured skin fibroblasts by Trizol and treated with RNase-free DNase (Life Technologies Europe, Ghent, Belgium). .. For the conversion to cDNA, Moloney murine leukemia virus reverse transcriptase was used in combination with random hexanucleotide primers (Invitrogen, Life Technologies Europe). .. gDNA was isolated from fibroblast cultures by the Easy-DNA kit (Invitrogen) or extracted from blood samples using the Qiaquick kit (Qiagen, Hilden, Germany).

    Isolation:

    Article Title: RNA Binding Protein CUGBP2/CELF2 Mediates Curcumin-Induced Mitotic Catastrophe of Pancreatic Cancer Cells
    Article Snippet: Results were analyzed with ModFit LT software (Verity Software House, Topsham, ME). .. Total RNA isolated from MiaPaCa-2, Pan02 cells and tumor xenografts using TRIZOL reagent was reverse transcribed with Superscript II reverse transcriptase in the presence of random hexanucleotide primers (all from Invitrogen, Carlsbad, CA). .. Complementary DNAs were then used for Real time PCR using Jumpstart Taq DNA polymerase (Sigma Chemical Co, St. Louis, MO) and SYBR Green nucleic acid stain (Molecular Probes, Eugene, OR).

    Article Title: Nanoparticle-based delivery of siDCAMKL-1 increases microRNA-144 and inhibits colorectal cancer tumor growth via a Notch-1 dependent mechanism
    Article Snippet: .. Total RNA isolated from tumor xenografts and HCT116 cells was subjected to reverse transcription using SuperscriptTM II RNase H-Reverse Transcriptase and random hexanucleotide primers (Invitrogen, Carlsbad, CA). .. The complementary DNA (cDNA) was subsequently used to perform real-time polymerase chain reaction (PCR) by SYBRTM chemistry (SYBR Green I, Molecular Probes, Eugene, OR) for specific transcripts using gene-specific primers and JumpStartTM Taq DNA polymerase (Sigma-Aldrich).

    Quantitative RT-PCR:

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals.
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [31], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [28,31]. ..

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [ ], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [ , ]. ..

    cDNA Synthesis:

    Article Title: Lipocalin-2 Deficiency Diminishes Canonical NLRP3 Inflammasome Formation and IL-1β Production in the Subacute Phase of Spinal Cord Injury.
    Article Snippet: Afterwards, purity and RNA concentration were measured using a NanoDrop 1000 device (PeqLab). .. For cDNA synthesis, 1 μg of RNA was used, and reverse transcription was performed using an MMLV reverse transcriptase kit and random hexanucleotide primers (Invitrogen, Braunschweig, Germany). .. Semiquantitative real-time PCR (qRT-PCR) was performed using SYBR Green (Bioline, Braine-Álleud, Belgium, QT615-05) in the CFX Connect qPCR detection system (Bio-Rad, Feldkirchen, Germany).

    Article Title: Small molecule kinase inhibitor LRRK2-IN-1 demonstrates potent activity against colorectal and pancreatic cancer through inhibition of doublecortin-like kinase 1
    Article Snippet: .. First strand cDNA synthesis was carried out using SuperScript II Reverse Transcriptase and random hexanucleotide primers (Invitrogen). .. The complementary DNA was subsequently used to perform RT-PCR on an iCycler IQ5 Thermal Cycler (BioRad) using SYBR Green (Molecular Probes) with gene-specific primers and JumpStartTM Taq DNA polymerase (Sigma).

    Virus:

    Article Title: Ehlers-Danlos Syndrome, Hypermobility Type, Is Linked to Chromosome 8p22-8p21.1 in an Extended Belgian Family
    Article Snippet: Total RNA was isolated from cultured skin fibroblasts by Trizol and treated with RNase-free DNase (Life Technologies Europe, Ghent, Belgium). .. For the conversion to cDNA, Moloney murine leukemia virus reverse transcriptase was used in combination with random hexanucleotide primers (Invitrogen, Life Technologies Europe). .. gDNA was isolated from fibroblast cultures by the Easy-DNA kit (Invitrogen) or extracted from blood samples using the Qiaquick kit (Qiagen, Hilden, Germany).



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